Uporedno ispitivanje citoloških, histopatoloških i imunohistohemijskih metoda dijagnostike kutanih i supkutanih masa kod pasa

In this study, we compared the cytological, histopathological, and immunohistochemical diagnoses of 71 canine cutaneous and subcutaneous masses. Cytological diagnoses included 56 tumors (21 mesenchymal, 15 epithelial, 16 round cell, four melanocytic), 13 infl ammatory reactions, and two cysts. Of the 21 cytologically diagnosed mesenchymal tumors, three were later confi rmed non-tumoral (hematoma, granulation tissue, fi broepithelial polyp). Thirteen out of 15 epithelial tumors were correctly diagnosed cytologically, whereas two cases were confi rmed to be non-tumoral (fi broepithelial polyp, granulation tissue) after histopathological examination. One mast cell tumor was later confi rmed as fi brous hyperplasia; diagnoses were correct in other round cell tumors. Cytological diagnoses were correct for all melanocytic tumors and cystic lesions. Five cases which had been cytologically diagnosed as infl ammatory reactions were diagnosed as tumors (lymphoma, papilloma, sebaceous adenoma, and squamous cell carcinoma) after histopathological examination. Immunohistochemistry confi rmed the histopathological diagnoses of all epithelial and round cell tumors, while the diagnoses of six mesenchymal tumors were changed after the immunohistochemical examination. The total accuracy of cytology in the diagnosis of tumoral/non-tumoral masses was 84.5%, and the accuracy in the determination of benign/malignant behavior was 83%. Diagnostic accordance between histopathology and immunohistochemistry was 86.6%. High success rates obtained with cytological diagnoses prove that cytology is a reliable diagnostic tool. The main diagnostic challenge remains with mesenchymal tumors and tumors accompanied by infl ammatory reactions. The results suggest that immunohistochemistry is fundamental for diagnoses of most mesenchymal tumors.U studiji je urađena uporedna analiza citoloških, histopatoloških i imunohistohemijskih dijagnostičkih metoda ispitivanja kutanih i supkutanih masa kod 71 pasa. Citološka dijagnoza je obuhvatala 56 tumora (21 mezenhimskog porekla, 15 epitelnih, 16 tumora okruglih ćelija i četiri melanocitna tumora), 13 infl amatornih promena i dve ciste. Od dvadesetjednog mezenhimskog tumora, tri su kasnije potvrđena kao neneoplastične lezije, (hematomi, granulomatozno tkivo, fi broepitelijalni polip). Posle histopatoloških ispitivanja, trinaest od ukupno 15 epitelnih tumora, citološki su bili pravilno dijagnostikovani pri čemu su dva potvrđena kao neneoplastične tvorevine (fi broepitelni polip, granulaciono tkivo). Jedan mastocitom je kasnije potvrđen kao fi brozna hiperplazija. Dijagnoza je bila tačna u slučajevima tumora okruglih ćelija. Citološka dijagnoza je bila tačna u svim slučajevima melanotičnih tumora i cista. Pet slučajeva kod kojih je citološki nalaz ukazivao na zapaljenske reakcije, posle histopatološkog ispitivanja dijagnostikovani su kao tumori (limfom, papilom, sebaceozni adenom i skvamozni karcinom). Primenom imunohistohemijskih metoda, potvrđene su histopatološke dijagnoze svih epitelnih i tumora okruglih ćelija dok je dijagnoza šest mezenhimskih tumora promenjena posle ispitivanja imunohistohemijskim metodama. Tačnost citoloških ispitivanja u dijagnostici tumora i netumorskih tvorevina bila je 84,5%, pri čemu je tačnost u određivanju benignog/malignog ponašanja tkiva bila 83%. Usklađenost između histopatološkog i imunohistohemijskog rezultata bila je 86,6%. Visok nivo tačnosti koji je dobijen na osnovu citoloških ispitivanja, dokazuje da je citološka metoda ispitivanja dobra početna dijagnostiča tehnika. Međutim i dalje je glavni izazov u dijagnostičkom smislu, ispitivanje mezenhimskih tumora kao i tumora praćenih infl amacijom. Rezultati ukazuju da imunohistohemijsko ispitivanje predstavlja osnovu dijagnoze većine mezenhimskih tumora

Improved classification, diagnosis and prognosis of canine round cell tumours

As the name suggests, canine round cell tumour (RCTs) are composed of cells with a round morphology. There is some discrepancy amongst authors as to which tumours belong to this category, but most designate lymphomas, melanomas, plasmacytomas, transmissible venereal tumours (TVTs), histiocytomas, and mast and neuroendocrine cell tumours

Comparative Evaluation of the Cytological, Histopathological and Immunohistochemical Findings of Canine Cutaneous and Subcutaneous Masses

In this study, we compared the cytological, histopathological, and immunohistochemical diagnoses of 71 canine cutaneous and subcutaneous masses. Cytological diagnoses included 56 tumors (21 mesenchymal, 15 epithelial, 16 round cell, four melanocytic), 13 infl ammatory reactions, and two cysts. Of the 21 cytologically diagnosed mesenchymal tumors, three were later confi rmed non-tumoral (hematoma, granulation tissue, fi broepithelial polyp). Thirteen out of 15 epithelial tumors were correctly diagnosed cytologically, whereas two cases were confi rmed to be non-tumoral (fi broepithelial polyp, granulation tissue) after histopathological examination. One mast cell tumor was later confi rmed as fi brous hyperplasia; diagnoses were correct in other round cell tumors. Cytological diagnoses were correct for all melanocytic tumors and cystic lesions. Five cases which had been cytologically diagnosed as infl ammatory reactions were diagnosed as tumors (lymphoma, papilloma, sebaceous adenoma, and squamous cell carcinoma) after histopathological examination. Immunohistochemistry confi rmed the histopathological diagnoses of all epithelial and round cell tumors, while the diagnoses of six mesenchymal tumors were changed after the immunohistochemical examination. The total accuracy of cytology in the diagnosis of tumoral/non-tumoral masses was 84.5%, and the accuracy in the determination of benign/malignant behavior was 83%. Diagnostic accordance between histopathology and immunohistochemistry was 86.6%. High success rates obtained with cytological diagnoses prove that cytology is a reliable diagnostic tool. The main diagnostic challenge remains with mesenchymal tumors and tumors accompanied by infl ammatory reactions. The results suggest that immunohistochemistry is fundamental for diagnoses of most mesenchymal tumors.U studiji je urađena uporedna analiza citoloških, histopatoloških i imunohistohemijskih dijagnostičkih metoda ispitivanja kutanih i supkutanih masa kod 71 pasa. Citološka dijagnoza je obuhvatala 56 tumora (21 mezenhimskog porekla, 15 epitelnih, 16 tumora okruglih ćelija i četiri melanocitna tumora), 13 infl amatornih promena i dve ciste. Od dvadesetjednog mezenhimskog tumora, tri su kasnije potvrđena kao neneoplastične lezije, (hematomi, granulomatozno tkivo, fi broepitelijalni polip). Posle histopatoloških ispitivanja, trinaest od ukupno 15 epitelnih tumora, citološki su bili pravilno dijagnostikovani pri čemu su dva potvrđena kao neneoplastične tvorevine (fi broepitelni polip, granulaciono tkivo). Jedan mastocitom je kasnije potvrđen kao fi brozna hiperplazija. Dijagnoza je bila tačna u slučajevima tumora okruglih ćelija. Citološka dijagnoza je bila tačna u svim slučajevima melanotičnih tumora i cista. Pet slučajeva kod kojih je citološki nalaz ukazivao na zapaljenske reakcije, posle histopatološkog ispitivanja dijagnostikovani su kao tumori (limfom, papilom, sebaceozni adenom i skvamozni karcinom). Primenom imunohistohemijskih metoda, potvrđene su histopatološke dijagnoze svih epitelnih i tumora okruglih ćelija dok je dijagnoza šest mezenhimskih tumora promenjena posle ispitivanja imunohistohemijskim metodama. Tačnost citoloških ispitivanja u dijagnostici tumora i netumorskih tvorevina bila je 84,5%, pri čemu je tačnost u određivanju benignog/malignog ponašanja tkiva bila 83%. Usklađenost između histopatološkog i imunohistohemijskog rezultata bila je 86,6%. Visok nivo tačnosti koji je dobijen na osnovu citoloških ispitivanja, dokazuje da je citološka metoda ispitivanja dobra početna dijagnostiča tehnika. Međutim i dalje je glavni izazov u dijagnostičkom smislu, ispitivanje mezenhimskih tumora kao i tumora praćenih infl amacijom. Rezultati ukazuju da imunohistohemijsko ispitivanje predstavlja osnovu dijagnoze većine mezenhimskih tumora

Long term dietary restriction ameliorates swimming exercise-induced oxidative stress in brain and lung of middle-aged rat

24-31Exhaustive exercise may generate oxidative stress in brain and reported findings are conflicting. Long term dietary restriction (DR) may be useful in the inhibiting of free oxygen radicals generated during exhaustive exercise in the brain of rat. Hence, in this study we evaluated beneficial effects of long term DR on the oxidative stress and antioxidant enzyme systems in brain cortex and lung in rats after different intensities of swimming exercise. Sprague-Dawley rats (60) were assigned as DR and ad libitum (AL) groups, and each group was further subdivided into three groups namely control (sedentery), submaximal exercise (endurance exercise) and maximal exercise (exhaustive swimming exercise) groups. Animals in the endurance exercise group swam 5 days/week for 8 weeks while exhaustive swimming group was subjected to an acute bout of exercise. With the increase in intensity of exercise, degree of lipid peroxidation (LP) and protein oxidation (PO) were also increased in DR and AL groups; however rate of increase was lower in DR group than AL group. Glutathione (GSH) and glutathione peroxidase (GSH-Px) activity were lower but glutathione reductase (GR) activity was higher in DR group compared to AL group in endurance and exhaustive swimming exercise. With increase in exercise intensity, GSH and GR enzyme activity decreased, whereas an increase was observed in GSH-Px enzyme activity. There was no difference in LP, PO, GSH and GR activity between DR and AL groups. GSH-Px activity in brain cortex was significantly lower in DR group than in AL group and sedentary rats. Results indicate that long term dietary restriction may protect against endurance and exhaustive swimming exercise-induced oxidative stress in rats by inhibiting oxidative stress

The effects of a low dose of cabergoline on induction of estrus and pregnancy rates in anestrous bitches

This is the first report of successful induction of normal estrus and ovulation in breeder bitches with as a low dose as 0.6 mu g/kg/day of cabergoline formulation marketed for use in women. Sixty-one pure breed bitches from various breeds were used in the study at their already determined periods of anestrus. Twenty-four dogs formed the control group, while 37 bitches were administered with two different doses of cabergoline (recommended dose group, n = 10, 5 mu g/kg/day and low dose group, n = 27, 0.6 mu g/kg/day). Induced estrus rates and mean treatment and proestrus durations of dogs in these two dose groups were compared

Protective effects of long term dietary restriction on swimming exercise-induced oxidative stress in the liver, heart and kidney of rat

In this study, we evaluated the hypothesis that long term dietary restriction would have beneficial effects on the oxidative stress and antioxidant enzyme systems in liver, heart and kidney in adult mate rats undergoing different intensities of swimming exercise. Sixty male, Sprague-Dawley rats were assigned as either dietary restricted on every other week day (DR) or fed ad libitum (AL) groups, and each group was further subdivided into sedentary, endurance swimming exercise training (submaximal exercise) and exhaustive swimming exercise (maximal exercise) groups. Animals in the submaximal exercise group swam 5 days/week for 8 weeks, while maximal exercise was performed as an acute bout of exercise